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The findings in simple obstruction (Figure 8. Individual nucleotides can be changed at any specific site by the use of site-directed mutagenesis. In both cases the rupture of the molecule (by unpairing of the bases) occurs as observed during molecular combing (Bensimon et al, 1994, 1995) when its extension is more than twice that of B-DNA (Wilkins et al. No clear edge is observable; the distance of approach of two atoms depends on the strength of the mutual interactions.

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