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Transmembrane pH and Ammonium Ion Gradients Three different approaches were used: Use of the pH-sensitive membrane-impermeable flurophore pyranine based on the ratiometric method, which determines directly level of dissociation of pyranine from the ratio between the charged (unprotonated) pyranine and total pyranine in the intraliposome aqueous phase: Addition of impermeable DPX, which acts as a quencher to pyranine fluorescence, into the liposome external medium ensures lack of contribution of extraliposome medium pyr- anine fluorescence (18,22).

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Notice the two chains joined by one cross-link, the inter- nal cross-link in the black chain, the attachment of the initiator (RS) to the green chain, and the (E,E)-dienes in both chains.

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116119. Often, if you dont pay this much attention, you will revert back to isolated analysis and decision-making. 131. acid-resistant and minimum odour grades, may have somewhat higher shrinkage values. European Journal of Clinical Investigation 12, 3519. This diversity, however. 488 This statement is for an infinite allele mutation model and may not be appro- priate for a stepwise mutation model.

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Upon completion of the reaction, the solvent was removed at reduced pressure, and the sodium salt were precipitated with addition of 400 binafy of ether.

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Column 3 shows the electric multipolar moment needed to produce the same primary field in the limit as the ratio of sphere size to wave length becomes vanishingly small, and Column 4 shows the number of separate charges necessary to produce each static multimoment.

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